10 20
30 40 50
60 70 80
90
100
110
120
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum MSVLKGALAVLMKQEVLTLGEYLEQKRKIRVFKEEGLYNDSSGELRRLLEETLILEDPSIPKALKEGFLGLRELTFNSLDNSLDINGDRRKLLGINIEKLLEWSSILGQNYFSSPRNQSS
Cryptosporidium
hominis MSVLKGALAVLMKQEVLTLGEYLEQKRKIRVFKEEGLYNDSLGELRRLLEKTLILEDPFMPKALKEGFLGLRELTFNSLDNSLDINGDRRKLFGINIEKLLEWSSILGQNYFSSPRNQSS
Giardia
lamblia ------------------------------------------------------------------------------------------------------------------------
Giardia
intestinalis ------------------------------------------------------------------------------------------------------------------------
Leishmania
amazonensis -----------------------------------------------------------------------------------------MSASFPSIPGFAGPLSLKAFLEEYFGLHLTF
Leishmania
infantum -----------------------------------------------------------------------------------------MSASFPSIPGFAGPLSLKAFLEEYFGLHLTF
Trypanosoma
brucei -----------------------------------------------------------------------------------------MAQMFPSVPGYDGPFSLKDFLHNYIGLQLRW
130
140
150
160
170
180
190
200
210
220
230
240
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|-------------------------------------------------------------------------------------------------Mo
Cryptosporidium
parvum MSSGKRSRNTEYNETLSPSFITD-QIKNIIPDSEKDS-----------------------------------------------------LTYQVQDFELLLRK--HFPRIRIFHS-GSY
Cryptosporidium
hominis MSSGKRSRNNEYNETLPPSFITD-QIKNIIPDSEKDS-----------------------------------------------------PTYQVQDFELLLRK--HFPRIRIFHS-GSY
Giardia
lamblia --------------------MDFPLSLLDANVSKAIF-----------------------------------------------------ILLQGEPEQASLWS--LILQLRTPGQ-GSS
Giardia
intestinalis --------------------MDFPLSLLDANVSKAIF-----------------------------------------------------ILLQGEPEQASLWS--LILQLRTPGQ-GSS
Leishmania
amazonensis AAETASPSPRAAATAETPSAAG-FRALRDVVLPPNQSFLVVVYVALHASSSPPPTTAHASPTPATPDPGCAAPPTGLGRLRQPLAHQTAVSTAHDTCVTRCNPSDRQNPFCLSSSSTGKN
Leishmania
infantum AVETASPSPRAAAAAEAPSAAG-FRALRDVVLPPNQSFLVVVYVALHASSSPPPTTAHASPTPPTPAPGCAASATGFGRLRQPLTHQTAASTAHDTYVARCSPADRPNSSCSSSSSTGKN
Trypanosoma
brucei STPQENSTTLPPSNSN-----------VVVVPPTGNFHVVVYVSRSAPPP----------------------------------QRSVRSVAVLSSC----------------------S
250
260
270
280
290
300
310
320
330
340
350
360
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
tif GQ--------------------------------------------------------------------------------------------|
Cryptosporidium
parvum SGLRTSFDTIKPKKYSFCN---STLYKFQ-NLHAWNT-----LLSKI-GPIEILFLFVCCIIFRILGDHSE-ILIQQAGRMLTNDFLEELARLYETGPK--KTNSFVSSSSLTPILTIEE
Cryptosporidium
hominis SGLRTSFDTIKHKKYSFCN---STSYKFQ-NLHAWNT-----LLSKI-GPIEILFLFVCCIIFRILGDHSE-ILIQQAGRMLTNDFLEELARLYETGPK--KTNSFVSSSSLTPILTIEE
Giardia
lamblia FASCA-----------------SLIYKDLKGLSSWIT-----EAAFI-PFLRRLGFCSLYLSYG---HDVL-LELFKYPIVLTSTVSGKRVLLSEPQMP---------------------
Giardia
intestinalis FASCA-----------------SLIYKDLKGLSSWIT-----EAAFI-PFLRRLGFCSLYLSYG---HDVL-LELFKYPIVLTSTVSGKRVLLSEPQMP---------------------
Leishmania
amazonensis GNARSPWCASASWLLYTNTSHRPFTDALLRHP--WRASFSACLGPAAMGFIE-MY----CPIVLQLEAMAGGVQVIGPALKHVALETSFSATAQLSEMKGLPPRGSASVSSRGVKRAVDT
Leishmania
infantum GNARGLWRSSAFWLLYTNTSHRPLTDALLRHP--WWASFAACLGPAAMGFIE-MY----CPIVLQLEAMAGGVQVLGPALKHAALETSFSATAPSSEMKGPPPQGSGYVSSRGVKRAVDT
Trypanosoma
brucei NKMDG---DHPQPSVPTHFHGRPLTSSVLKHP--FWDALFSQIGPTAVSFIV-LW----APIVVQFEASGGGLQVLGPPLKR-----------------SASCAVKRQPGAG--------
370
380
390
400
410
420
430
440
450
460
470
480
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum KEEVKPLDETPRQVNKRSGGIIKDSRLSKIYKIDIPYRSTFLYCDHFSKRGGLPCLSVLRLLPPNLLGARTLLRFIIQTDHIFKDHNRQSLIGLLGSYEMTKFSRVKCKLASAMMEEFQN
Cryptosporidium
hominis KEEVKPLDETPRQVNKRSGGIIKDSRLSKIYKIDIPYRSTFLYCDHFSKRGGLPCLSVLRLLPPNLLGARTLLRFIIQTDHIFKDHNRQSLIGLLGSYEMTKFSRVKCKLASAMMEEFQN
Giardia
lamblia ---------------------------------------------------------------LAFPPCLHDKSVSSMVRASTVQYASNCLEEFISMIQEHPMNVRFIFFCKTRPYIRAF
Giardia
intestinalis ---------------------------------------------------------------LAFPPCLHDKSVSSMVRASTVQYASNCLEEFISMIQEHPMKLRFIFFCKTRPYIRAF
Leishmania
amazonensis GE-CSAPLPLQKQRRVEAVASPPKAGRRLQREVAPSHRPCRDDSFSSPVNRAAMPATWAAALTRTDDPRTRLYSVRVSDSDCTGDG-GGALPLPAGSLEAHWLPRHPRSLHRVLQAALPK
Leishmania
infantum GE-CSGFLPLQKQRRVEAPP-KAKAGRRLQRGVVPCSRPRRDDPFASPVNRAAMPVTWAAALTRTDVPRTRLYSVRVSDSDGAGDGSGGALPLPAGFLEAQWLRRHPHSLHRVLQAALPK
Trypanosoma
brucei ---WWEGKLKVSRTETLTQND---------------------------PHCFCIEGTSSPVALYRLNVPRLHLVEQSPLRST------ADARHAVTLILEQLWIEHHKSSCSPSSDRDIS
490
500
510
520
530
540
550
560
570
580
590
600
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------------Motif CP---------- -----
Cryptosporidium
parvum L--------------------------------------------------------------------------------LLNI------------RN-TSP-VN-FLD---QI-CPIE
Cryptosporidium
hominis L--------------------------------------------------------------------------------LLNI------------RN-TSP-VD-FLG---QI-CPIE
Giardia
lamblia HSLALLGSATAIWHLSLLPSQCLTAFYQANPLVTFKFPYNAFSTRSFSSLLQLCTSRGRLMPNNAINSNAKARITRLISIFRSKM------------KR-LSHNLT-FL----KD-KKPQ
Giardia
intestinalis HSLALLGSATAIWHLSLLPSQCLTAFYQANPLVTFKFPYNAFSTRSFSSLLQLCTSRGRLMPNNAINSNAKARITRLISIFRSKM------------KR-LSHNLT-FL----KD-KKPQ
Leishmania
amazonensis RAAYGASTRHYCAGTGERGTGCTSVKNISMWHVAHVFRWLV----------------------------------------LQPSQSGEVAFQTTSPKFDLPSYLRRFLSTDVDQ-CSRL
Leishmania
infantum RVAYGASARRYSVGASERVTGCTSVEDIPMWHVAHVFRWLV----------------------------------------LQPPQSGADASPTTPPKFDLPSYLRRLLSTVVDQ-CSRL
Trypanosoma
brucei QRLCG------VATGSATNCCGDSCVVLAQARNQVNAV-------------------------------------------FPYTL-LPVHDDAVSGNGTVKEYLIHVLHCALGSVC-RM
610
620
630
640
650
660
670
680
690
700
710
720
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
| |--------------------------Motif QFP-------------------------------
Cryptosporidium
parvum PIKDSDLQNFNKL----------------------------------------PIPCFETSSTRVVNF-------LRLYLIKVLPKNILGTFKNFKTFINKKIPIIVNLHIRETFKIQHA
Cryptosporidium
hominis PIRDSDLQNFNKL----------------------------------------PIPCFETSSTRVVNF-------LRLYLIKVLPKNILGTFKNFKTFINKKIPIIVNLHIRETFKIQHA
Giardia
lamblia SLLQSFGFDTHFRVFLDE-----------TGTL--------------------RHAPLPLSVHKITLY-------LMLYCKSISAFSILG-KRNARTFH-SHLFRFISQPMNGFMTLQEL
Giardia
intestinalis SLLQSFGFDTHFRVFLDE-----------TGTL--------------------RHAPLPLSVHKITLY-------LMLYCKSISAFSILG-KRNARTFH-SHLFRFISQPMNGFMTLQEL
Leishmania
amazonensis DLRGAALRHTGYLEEAFR--------RQQQGVEPWDVQRL---STPVDVVVS--YLRTLLPTLRWAPL-------KEANNGLFWGRDAAGSERVLDALM-RAVRGWLIAGRQAVVPVSRF
Leishmania
infantum DLRGAALKHTGYLEEAFR--------RQQQGVEPWDVQRL---STPVDVVVS--YLRTLLSTLRWAPL-------KEAKNRSFWGRDAAGSERVLDALM-GAVRNWLIAGRHAIVPVSRF
Trypanosoma
brucei NIRGAAIKHTEFLEKRGKSVTSISQKAQTAGQENKMRAIPVGELTVPEPVVAS-YLETLFNMMWWRVPPRFVGGAVTADRVKFWGEEGT----VLTRLR-EVTVDWLCCGRHEVFLLSHF
730
740
750
760
770
780
790
800
810
820
830
840
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
------------| |----------------------Motif T------------------------
Cryptosporidium
parvum MNGIEVSN--WVNRILEEKSYQFIKESKNLINSNQRSKKVPNNKTRSSIKKSLISLGKTYLARNIYFL-IVYLVFP-----ILRRHF----YATEIE-GSNKV-----RYFRHPVWIKIV
Cryptosporidium
hominis MNGIEVSN--WVNRILEEKSYQFIKESKNLINSNQRSKKVPNNKTRSSIKKSLISLGKTYLARNMYFL-IVYLVFP-----ILRRHF----YATEIE-GSNKV-----RYFRHPVWIKIV
Giardia
lamblia TSSISTKEF--L-LPATTVSKAEGEVRHHLLAYFVV------------------------------YL-MDFVILP-----ALYQSFSAVSMHTELSNRIETANSMVPALLSRSLWVSYK
Giardia
intestinalis TSSISTKEF--L-LPATTVSKAEGEVRHHLLAYFVV------------------------------YL-MDFVILP-----ALYQSFSAVSMHTELSNRIETANSMVPALLSRSLWVSYK
Leishmania
amazonensis LDGVPVAQVPWL-NGFYTTTPSLPFSDATSSASRHARRERSQVQQRV-------------------WLQFVLFLTQDILPFLLRASF----TITWSSKNTHKL-----LFFPAFVWRRLV
Leishmania
infantum LDGVPVAQVPWL-NGFYTTTPSLPFSAATSSAARHARRERSHVQQRV-------------------WLQFVLFLTQDILPFLLRASF----TITWSSKNTHKL-----LFFPAFVWRRLI
Trypanosoma
brucei LDGVPVSRIPWL-RGFYTKEP-----------ARKRRSMIQQRV----------------------FLQLVLFLYQCVVPFLIRRSF----HVACTSKSPYIF----FFIPRAVWVRLTR
850
860
870
880
890
900
910
920
930
940
950
960
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
--------| |--1-||---2--|
Cryptosporidium
parvum RQADKWYLESILKGIQSKDYIMNLSSIQEISKLIDKNSEFSENIPK-------------------------------------------------------IRWVPKSKGLRPLINLSKV
Cryptosporidium
hominis RQADKWYLESILKGIQSKDYIMNLSSIQEISKLIDKNSEFSENIPK-------------------------------------------------------IRWIPKSKGLRPLINLSKV
Giardia
lamblia NYLIKSSARLEIAATYEFVCSLSNLHSIPLTNEDAVNRIFWKQVSPGR-----------------------------------------------------LSFVYKSNGRVRPLCNFSF
Giardia
intestinalis NYLIKSSARLEIAATYEFVCSLSNLHSIPLTNEDAVNRIFWKQVSPGR-----------------------------------------------------LSFVYKSNGRVRPLCNFSF
Leishmania
amazonensis RREVRRTRSCHAPRSQMSLAEERTRMSGADHAALVSAPNACGGAVAAAFPSPHSAASNASAAISAPRDEWRAVRTGGALAHWSARATLATRGGGASCLYAGVRFRPDRRKLRPIAVVRSA
Leishmania
infantum RREVRRTRSCGAPRSQMPLAGESARSVGAERVALSSAPCACEGAATAALPTPHSAASKASAAISAPRDVWRAVRTDGVLAHRGARATLATRGGGASCLYASVRFRPDRRKLRPIAVVRSA
Trypanosoma
brucei REMRRVCIRRVKRMRSDTLSDPQMRGHAGLPPMALERVTSEGLMQLIGPSSQPVGDDS----------GGVSDVCKLRDSSSPLLYSD-------------VRFLVDGSKLRPIARPRFG
970
980
990
1000
1010
1020
1030
1040
1050
1060
1070
1080
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum GSGQILQQNLERDHTCEEKKKIGFCDCNSVWTGGDLPTSWINNNYYYNYFNSNGNNGIVGNNHNYGHINNIGGKNFTSFSSQNCQIMTPPGRLGVGLGFGNYNIGIGINSGSSVPTTPIN
Cryptosporidium
hominis GSGQILQQNLERDHTCEEKKKIGFCDCNSVWTGGDLPTSWINNNYYYNYYNSNGNNGTVGNNHNYGHINNIGGKNFTSFSSQNCQIMTPPGRLGVGLGFGNYNIGIGINSGSSVPTTPIN
Giardia
lamblia CSRRHRVS----LNSFLRSALQVIIFELERP-------------RNRYLQAHLAKNLGHIAVDY-----------AAWLDATY------TENPE--------------------------
Giardia
intestinalis CSRRHRVS----LNSFLRSALQVIIFELERP-------------RNRYLQAHLAKNLGHIAVDY-----------AAWLDATY------TENPE--------------------------
Leishmania
amazonensis SLRSLKEMARGSPSPYSHASAIVRLLRRLGCSDADGQLPAATATLLRQVQARSRHNRRTGVHRSAHPLPPHLPHKAALQDALRCLVSGVEEQRVRDGLPRLSNLSHQDEYAELRSFCEEV
Leishmania
infantum SLRSLKEMARGSPSPYSHASAIVRLLCRLGCSDTDGQLPAATATLLRRVQARSRHNHHTGVHRFPHPLPPHLPHKAALRDALRCLVSGVEEQRVRDGLPRLSNLSHQDEYAELRSFCEEV
Trypanosoma
brucei WQRSLLKAADG---------------------------VGSSTLASALAKAGKYLATDGKAMWPS-------PNSAVLRDALRCLDAGVEER----------RITTGARQRAIGSHNDEY
1090
1100
1110
1120
1130
1140
1150
1160
1170
1180
1190
1200
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------------Motif A-------------
Cryptosporidium
parvum KVMRRPSSNRIIDIRRPSTNNMLFYPSKILRSFLLRMTGKNHLGASLVQFGDIYKIIKNWWIKNRRDKQQFQMNEDFYQNQGKKMSHVK---IYIIKADLVNCFENINKSKIFEFLD-VI
Cryptosporidium
hominis KVMRRPSSNRIIDIRRPSTNNMLFYPSKILRSFLLRMTGKNHLGASLVQFGDIYKIIKNWWIKNRRDKQQFQMNEDFYQNQGKKMSHVK---IYIIKADLVNCFENINKSKIFEFLD-VI
Giardia
lamblia ------------------------------------------------------------------------------------------AQIYMVTTDMITAFESVSVPRLLYYISHYI
Giardia
intestinalis ------------------------------------------------------------------------------------------AQIYMVTTDMITAFESVSVPRLLYYISHYI
Leishmania
amazonensis RGRHAIPCEGPAKTTPAVSSASPPGA-----------------------------------------------------------TACFAPYVTLLRSDASRCYDNLPQGRVLAAVRSLV
Leishmania
infantum RRRPALPCEGPAGTAPVVSSASPPSAA----------------------------------------------------------ACCFAPYVTLVRSDASRCYDNLPQERVLAAVASLV
Trypanosoma
brucei IEVRSFIQCARQFCTIPTLVQGETSAA----------------------------------------------------------LLADATAVTMVRGDAARCYDHLPQEAVVNIMRGLV
1210
1220
1230
1240
1250
1260
1270
1280
1290
1300
1310
1320
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
---|
Cryptosporidium
parvum SLPNEISLLSLYSRALSKTTIIPPFDNMNRDSYQDELGRSCIITSKGKLNMVPIFEKDHDEDQKVVKSKIKRIIGPDLDELLWNLKNSCLSNKDLSILGQKKAEIFTFLNSRRVINWKLV
Cryptosporidium
hominis SLPNEISLLSLYSRALSKTTIIPPFDNMNRDSYQDELGRSCIITSKGKLNMVPIFEKDHDEDHKVVKSKIKRIIGPDLDELLWNLKNSCLSNKDLSILGQKKAEIFTFLNSRRVINWKLV
Giardia
lamblia VT--GNAYVVLTYKEIAPSKMPKIRTVALPCQHNGCVSMDSISRYLLCKETPKKQPNSMLCPMHARIYKREDIIK-----MLELHLLNP-------------------------------
Giardia
intestinalis VT--GNAYVVLTYKEIAPSKMPKIRTVALPCQHNGCVSMDSISRYLLCKETPKKQPNSMLCPMHARIYKREDIIK-----MLELHLLNP-------------------------------
Leishmania
amazonensis KH--DAYRVLRFTVIHAVDSEATCKGGCLLRRTFTTRTIPCAEAECGFLARIPRGHIYWEEE-GRTPT-GPHTSAAVSRTTDASSRCGANLISGAAVRA---------------------
Leishmania
infantum KH--DAYRVLRFTAIHALDGEAACKGGCLLRRTFTTRTIACAEAECGVLARIPRGHIYWEEEEEGRTLSGPHTTAAVSRTTKASSRCGANLISGAAVRA---------------------
Trypanosoma
brucei TH--ETYYSLKLTVVTLTTVKNVSKKT-----------RSSHAESQLSSMKETNGSTVKHRKLQLSKRVKLVPGRYVQEG-VLYGIARGCIAYEEQSVGSDSLVSGEEIRS---------
2µl
1330
1340
1350
1360
1370
1380
1390
1400
1410
1420
1430
1440
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|---------------------------Motif B'---------------------|
Cryptosporidium
parvum KEIIKIHLNTSFFRLRTCSKSLRLIKSKQLGNSIKFGKRFLSLFKQDFGIPQGSSISYILCCLYYNFLDLNPEIQNLLGHSFFSSSYISLSFLNPIKQEKVQLLDPTEKDLFSQHDLETG
Cryptosporidium
hominis KEIIKIHLNTSFFRLRTCSKSLRLIKSKQLGNSIKFGKRFLSLFKQDFGIPQGSSISYILCCLYYNFLDLNPEIQNLLGQSFFSSSCISLSFLNPIKQEKAQVLDPTKKDLFSQHDLETG
Giardia
lamblia --------------------------------LVIHEGGV---YRLTSGIPQGSVVSTVLFNCYSSLLHVQLLNTGIVSSRL--------------------------------------
Giardia
intestinalis --------------------------------LVIHEGGV---YRLTSGIPQGSVVSTVLFNCYSSLLHVQLLNTGIVSSRL--------------------------------------
Leishmania
amazonensis -----------------------LLSEHIRHHLVVVSGG--SLFEQRVGILQGSPVAMLLCDRLFSNVVDTALSSILSEHAER-------------------------------------
Leishmania
infantum -----------------------LLSEHLRHHLVVVSGG--NLLEQRVGILQGSPVAMLLCDRLFSDVVDTALSGILSEHAER-------------------------------------
Trypanosoma
brucei -----------------------VLQKHLQSHLVMLNG-K--MYIQRMGINQGSAVAMLLCDQLLERV-DAALSSILSEHEEP-------------------------------------
1450
1460
1470
1480
1490
1500
1510
1520
1530
1540
1550
1560
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|-----------Motif C-------------|
Cryptosporidium
parvum LSRYPEFKENEINIYNTSTSKKRRLEISEYNINAKSVLLQPSEEFNNNINNQNCQIRANLKSYDQESPSQSNKQESLLLRWVDDFLFLTSDLESAKKFLKLL-----YIQK-LWGSNVSK
Cryptosporidium
hominis LSRYPEFKENEINIYNTSTSKKRRLEISEYNINAKSVLLQPSEEFDNNINNQNCQIRVNLKNYDQESPSQSNKQESLLLRWVDDFLFLTSDLESAKKFLKLL-----YIQK-LWGSNVSK
Giardia
lamblia ---------------------------------------------------------------------------FFYRTFVDDWLLLTTSIALLDAYIEYLNIMRDHGAYFRLSCFTKP
Giardia
intestinalis ---------------------------------------------------------------------------FFYRTFVDDWLLLTTSIALLDAYIEYLNIMRDHGAYFRLSCFTKP
Leishmania
amazonensis ---------------------------------------------------------------------------SLLLRRVDDVLVATTSPAAAERCLRAM-----QRGWPSVGYLSNP
Leishmania
infantum ---------------------------------------------------------------------------SLLLRRVDDVLVATTSPAAAERCLQAM-----QRGWPSVGYLSNP
Trypanosoma
brucei ---------------------------------------------------------------------------ALLLRRVDDVLVVTLSRAAASRCEDAL-----RSGWSEIGFFCQE
|-------------------Motif
1570
1580
1590
1600
1610
1620
1630
1640
1650
1660
1670
1680
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
|--------------------Motif E------------------------|
Cryptosporidium
parvum DKINSNFPWIDHNNEIIILEEDDVLLSSP---------SSSCSSSCSSSSSSSSSSSSSS-SSSSSFPPSTE-CTSSPVEKRINTNKSEKDCQNEKEMINKAKIASKQFHKQVSWTGMKF
Cryptosporidium
hominis DKINSNFPWIDHNNEIIIFEEDDVLLSSP---------SSSCSSSCSSSSSSSS-------SSSSSFPPSTE-CLSSPVEERTNTSKGEKDCQNEKEMINKAKIALKQFHKQVSWTGMKF
Giardia
lamblia HKNSPFAPIATLDCSREPIPKKSLYTPNLNQDMYKLDVPRYCGYIFLEN-VAIIDVLKWFSSTRTNGSPSYPMIFGSGISVMQRLRLVFKKR----------------------------
Giardia
intestinalis HKNSPFAPIATLDCSREPIPKKSLYTPNLNQDMYKLDVPRYCGYIFLEN-VAIIDVLKWFSSTRTNGSPSYPMIFGSGISVMQRPRLVFKKR----------------------------
Leishmania
amazonensis SKLTLS------KACG---SLVP-----------------WCGLLLHDTTLEVSVEWRRIGVLLGSLRVGDPHYVHRGDYEPLYLTQRFLAVLQLRVA-PTALCGRMNSKTRQLQTFYEV
Leishmania
infantum SKLTLS------TACG---SLVP-----------------WCGLLLHDTTLEVSVEWRRIGVLLASLRVGDPHYVHRGDHEPLYLTQRFLAVLQLRVA-PTVLCGRMNSKTRQLQTFYEV
Trypanosoma
brucei EKLR-RV-----T-CG---QPVR-----------------WCGLLWDPVTLEFAVDWARLAKMMPYLAVRP--RTGCEPLLSSLRFIRILRLRTP----MTALCRQINSKSRVVQTLYEI
D------------------|
1690
1700
1710
1720
1730
1740
1750
1760
1770
1780
1790
1800
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum SSEYSYLNCMVSPWKNLEFICVMDTVTLTTKHQFTSNYSNFHRFKSTTISENLQKSNYMWSVLGIKLIRYFDFRIKNGLLYDCKINSLFTVSFKYNSTNNQPIN-
Cryptosporidium
hominis SSEYNYLNCMVSPWKNLEFICVMDTVTLTTKHQFTSNYSNFHRFKSMTISENLQKSNYMWSVLGIKLIRYFDFRIKNGLLYDCKINSLFTVSFKYNSTNKQPIN----------------
Giardia
lamblia -----------------------------------------IRDLRCFIERSIQAPDPRIKVDGSILYAYIRAVTFCILCYTRNIRYSESLRTLLRSTFCSLKATVWRHSTSRAIFIHVS
Giardia
intestinalis ---------IRDLRCFIERSIQAPDPRIKVDGSILYAYIRAVTFCILCYTRNIRYSESLRTLLR-----------------------STFCSLKATVWRHSTSRAIFIHVSKR-IILHVY
Leishmania
amazonensis GLLWTRVVLEKVQEALPVARNCCVTVLLLRPLAVCVGR----------------------------------------LCRLLSRHQRFLAARQSACDVSAAEVRACVLTALHRTVQAKL
Leishmania
infantum GLLWSRVVLEKVQEALPAARNRCVAVLLLHPLAVCVGR----------------------------------------LCRLLNRHQRFLAARKSACDVSAAEVRACVLTALHRTVQAKL
Trypanosoma
brucei GLLWSRFFLDKLKANAAFFR--PHVRTILQPLALATAT----------------------------------------LRRLVRKHSFDLQRLGSFCDVTDVEVRLCISAALYHTLHQRL
1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 1910 1920
....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|
structures
Cryptosporidium
parvum
Cryptosporidium
hominis -----------
Giardia
lamblia KRIILHVYGHNPRIRGLLLGMLSR--------------------------------------
Giardia
intestinalis GHNPRIRGLLLGMLSR----------------------------------------------
Leishmania
amazonensis RVLQARTVRAMTAQSGRTQRGPSLKGRRNNFCSTKASSVGRKGCEQRRRGRNRRN-TRVCLRSFWWLTAAEVESQWRRSLGALYRAAPRPGEACGSTASSPSPAASASLLMEDGPLSMHA
Leishmania
infantum RVLQARTVRVMTAQRGRTPRGTSLKGRQKPPCTNKASSAGCKYCERRRKGRNRRCSTRVCLRSFWWLAAAEVELQWRRSLGALHRAAPHPGEARGSTAPSLSPSASASLLMEDGPLSMHA
Trypanosoma
brucei AFMIGR---------------MSSVGKKKFLILMTAVLKRKMGELGSRINRNNQMESSLPSAADLLLVEGGDGVVSRGLAAVRFRPVETVRFQGCATRSA--------------------
1930
1940
....|....|....|....|
structures
Cryptosporidium
parvum
Cryptosporidium
hominis
Giardia
lamblia
Giardia
intestinalis
Leishmania
amazonensis RALSATRLSQT
Leishmania
infantum RALSATRLSQT
Trypanosoma
brucei -----------